Effect of 10 and 50 μM AMI on the use-dependent relationship of TTX-r Na
1.9 currents. A: The protocol of a single voltage step at −35 mV. B, C and D: Representative recordings of the Nav1.9 currents elicited by 60 pulses at 1 Hz before and after perfusion with 10 and 50 μM AMI, respectively. E: The effect of 10 and 50 μM AMI on the use-dependent relationship of Nav1.9 currents. Each point was normalized to the respective first Nav1.9 current (n = 9). F: The current at the 60th pulse normalized to the current of the first pulse, was not significantly decreased after perfusion with 10 or 50 μM AMI compared to that of the controls (n = 9, P > 0.05).