Representative double-staining of IL-6 mRNA by ISH and immunofluorescence for glial fibrillary acidic protein (GFAP). Detection of IL-6 mRNA in section of ipsilateral L4 DRG from sham-operated rat 14 d after surgery (A) and simultaneous immunofluorescence staining for GFAP (B). Signal for IL-6 mRNA was co-localized with GFAP immunofluorescence in SGC (C). The nuclei were labeled by Hoechst 33342. Arrows indicate position of SGC. Scale bars = 50 μm.