Figure 1
From: Sigma-1 receptor expression in sensory neurons and the effect of painful peripheral nerve injury
σ1 Receptor (σ1R) in various Control dorsal root ganglion (DRG) cell populations. (A) Staining for σ1R was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through co-staining with NeuN. No specific σ1R staining was found in neuronal nuclei. (B) σ1R was also found in cellular components identified as satellite glial cells by their expression of glutamine synthase (GS). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses σ1R. (C) Classification of control DRG neurons by size (<700, 700-1300 and >1300 μm3) showed greater σ1R intensity (arbitrary units, recorded using standardized image acquisition) in large neurons compared to others. (D) σ1Rs expression is compared in neuronal subtypes identified by their expression of calcitonin-gene related peptide (CGRP), isolectin B-4 (IB4), and neurofilament-200 (NF-200). (E) Higher fluorescent intensity for σ1Rs was found in the subpopulation of control neurons expressing NF-200. Mean ± SEM; number in bars represents the sample size; *p < 0.05, **p < 0.01, ***p < 0.001.