Figure 2
From: Sigma-1 receptor expression in sensory neurons and the effect of painful peripheral nerve injury
Effect of injury on σ1 Receptor (σ1R) expression in dorsal root ganglia (DRG). (A) Transcript measured by quantitative real time PCR showed no significant differences in σ1R level after spinal nerve ligation (SNL) in both the axotomized 5th lumbar (L5) dorsal root ganglion and the adjacent 4th lumbar (L4) ganglion, compared to control (C) ganglia. (B) Protein measurement by Western blot showed a dominant band at the expected molecular weight that was decreased in both L4 and L5 ganglia after SNL compared to control DRGs. β-Tubulin 1 (β-Tub) was used as loading control. (C) Summary data of Western blot showed a significant decrease of σ1R protein expression in axotomized L5 DRG. Both graphs show Median and interquartile range for fold difference from normalized controls; numbers in bars represents the sample size; **p < 0.01.