Figure 3

CFA-induced mechanical thresholds using von Frey filaments and incapacitance. A. Time to withdrawal measured using automated von Frey testing showed that both paws showed equal latency before CFA injection. Following injection, the time to withdrawal was significantly reduced in the injected paw compared to the contralateral paw, and the effect remained up to four days following CFA injection. B. Incapacitance testing was used to measure the ratio of weight-bearing between the two paws. This ratio went from 1 before CFA injection, indicating equal weight-bearing, to close to 0.5 following injection, indicating preference of the contralateral paw. This effect was measurable up to four days following CFA injection. C. Comparison of automated von Frey and incapacitance testing showed similar development of allodynia, which could be partially reversed by administration of CVID. No differences were seen between the two methods, therefore incapacitance testing was used in subsequent experiments in the interest of time.