Table 1 Summary of behavioral results showing main impact of G q -mediating sensitization processes
From: Gq rather than G11 preferentially mediates nociceptor sensitization
Ligand | Receptor | G-protein subclass | Thermal hyperalgesia – paw withdrawal latency | Mechanical allodynia – paw withdrawal response frequency | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
(Mean % change over pre-injection) | (Mean Δ increase over pre-injection) 0.4 g von Frey Filament | |||||||||
Control | G11-/- | SNS-Gq/11-/- | SNS-Gαq-/- | Control | G11-/- | SNS-Gq/11-/- | SNS-Gαq-/- | |||
Bradykinin | B | Gq/11 | -51.5 ± 3.3 (n=8) | -37.1 ± 2.5 (n=7) | -7.4 ± 11.5* (n=7) | -8 ± 2.7* (n=7) | 54.1 ± 5.5 | 27.8 ± 8* | 14.4 ± 3.6* | 6.7 ± 5.9* |
UTP | P2Y | Gq/11 | -44.2 ± 10.4 (n=8) | -23.8 ± 7.2 (n=8) | -9.7 ± 7.5* (n=7) | -9.8 ± 5.1* (n=8) | 32.8 ± 3.2 | 20 ± 8.9 | 4.8 ± 3.5* | 6.7 ± 6.0* |
Trypsin | PAR | Gq/11 | -41 ± 5.7 (n=8) | -38.3 ± 5 (n=8) | -17.7 ± 3.3* (n=8) | -14.3 ± 8.1* (n=6) | -3.3 ± 3.1 | 4.2 ± 1.8 | 0.8 ± 0.8 | |
CGRP | CGRP | Gq/11, Gs | -31,3 ± 6.9 (n=7) | -29.2 ± 2.3 (n=8) | 21.1 ± 8.7 | 6.7 ± 2.7 | ||||
ATP | P2Y | Gq/11, Gs | -48.4 ± 4.6 (n=8) | -42.2 ± 6.1 (n=7) | -16.7 ± 5.5* (n=8) | -12.3 ± 4.3* (n=8) | 27.5 ± 2 | 17.1 ± 6.5 | 4.8 ± 3.8* | 5 ± 2.4* |
ET-1 | ET | Gq/11, Gs, G12/13 | -39.5 ± 4 (n=8) | -42.3 ± 6.2 (n=7) | -21.7 ± 3.2* (n=8) | -25.5 ± 3.9* (n=7) | 26.7 ± 8.5 | 26.7 ± 5.7 | -0.8 ± 4.4* | 4.8 ± 5* |
PGE 2 | EP | Gq/11, Gs, Gi/o | -26.8 ± 5.6 (n=8) | -22.2 ± 8.8 (n=8) | -6.6 ± 8 (n=8) | -8 ± 5.7 (n=7) | 15.6 ± 5.8 | 7.5 ± 4.8 | -0.8 ± 2.9* | 4.8 ± 5* |
Serotonin | 5-HT | Gq/11, Gs, Gi/o | -41.9 ± 3.9 (n=8) | -34.4 ± 4.8 (n=8) | -31.4 ± 3.1 (n=8) | -24 ± 3.2* (n=7) | 29.2 ± 5.9 | 14.2 ± 5.1* | 7.5 ± 2.3* | 2.9 ± 2* |
Glutamate | mGluR1,2 | Gq/11, Gi/o | -45.1 ± 11.8 (n=9) | -8.2 ± 6.5 * (n=8) | -11.2 ± 4.5* (n=9) | -12.5 ± 6.7* (n=9) | 19.3 ± 9.7 | 30.4 ± 8.7 | 3.3 ± 6.3 | 6 ± 4.7 |
mcPAF | PAF | Gq/11, Gi/o | -49 ± 1.8 (n=7) | -38.7 ± 4.8 (n=8) | -30.7 ± 5 (n=7) | -26.7 ± 8.3 (n=8) | 30.5 ± 5.8 | 36.7 ± 7.4 | -1 ± 3.1* | 5.8 ± 5.6* |
Thrombin | PAR | Gq/11, Gi/o, G12/13 | -27.5 ± 6.2 (n=8) | -30.1 ± 5 (n=8) | 20 ± 3.3 | 16.7 ± 8.2 | ||||
S1P | S1P | Gq/11, Gi/o, G12/13 | -51.6 ± 5.7 (n=8) | -50.8 ± 5 (n=8) | -32.3 ± 4.5* (n=8) | -34.2 ± 7.3* (n=8) | 23.3 ± 5 | 20 ± 7.1 | 4.2 ± 2.5* | 0.8 ± 4.1* |