Figure 6

The effect of somatostatin in PrP-GFP neurons. a An example trace showing the membrane hyperpolarisation in response to somatostatin application. b The membrane potential before (control) and during somatostatin application. The mean hyperpolarisation was 8.9 ± 2.8 mV, and this was statistically significant (p < 0.05, n = 7). c The membrane potential did not change when somatostatin was applied in the presence of the sst₂ receptor antagonist, CYN 154806 (n = 5). d Currents measured in response to brief voltage steps (100 ms, -70 to -50 mV) in control and somatostatin conditions. e The current–voltage (I-V) relationship for the somatostatin-evoked current (n = 7). The reversal potential of this current is approximately -90 mV. f The membrane potential did not change when somatostatin was applied in the presence of the GIRK channel blocker, tertiapin-Q (n = 5), suggesting that the somatostatin-mediated membrane hyperpolarisation in the PrP-GFP cells involves activation of GIRK channels.