Figure 2
Comparison of the effects of the N-type VGCC blocker in the superficial layer and deep layer of the ACC. A, The same slice as Figure 1A. Two channels (Ch. 30 from LII/III and Ch. 28 from LV/VI) are selected for further comparison. B, ω-Ctx GVIA (1 μM) was bath applied for 15 min (filled circle: Ch. 30; open circle: Ch. 28) in a single slice. Sample fEPSP recordings taken at the times indicated by the corresponding numbers are shown above the plot. Calibration: 500 μV, 20 ms. C, Averaged data of 5–7 activated channels in the superficial layer (filled circle: LII/III) and deep layer (open circle: LV/VI) in one slice. D, Pooled data of 7 mice (LII/III: 45.8 ± 4.4% of baseline, LV/VI: 59.6 ± 2.8% of baseline, n = 7 slices/7 mice, t(12) = −2.663, P = 0.021). ω-Ctx GVIA produced a stronger inhibition of superficial layer responses than those of the deep layer in the ACC. The horizontal bars indicate the period of drug application. Asterisk indicates the statistical significance between superficial layer and deep layer of the ACC slice. Error bars represent SEM.