Figure 1

SNI mice exhibit prominent behavioral hypersensitivity. A) In response to dynamic stroke of a puffed cotton swab, SNI mice show significant desensitization of the tibial territory beginning POD 1 and continuing through POD 42 compared to sham or naïve animals (***p<0.005). At POD 49 SNI mice begin to regain sensation in the tibial territory, in that SNI mice still showed some sensitization compared to naïve animals (*p<0.05) but not sham animals (p>0.05). Treatments were compared across time using a repeated measure 2-way ANOVA with Tukey’s post hoc comparisons. B) SNI mice show a significant decrease in the 50% mechanical withdrawal threshold beginning POD 1 and continuing through POD 49 compared to sham or naïve mice (***p<0.005). Furthermore, SNI mice continue to show a significant decrease in mechanical withdrawal threshold at POD 90 compared to sham (***p<0.005) and at POD 180 compared to sham (*p<0.05). Treatments were compared across time using a 2-way ANOVA with Bonferroni post hoc tests. SNI and sham treatments were compared at POD 90 and POD 180 using Mann–Whitney U tests. C) In response to repeated stimulus with a 3.31 mN monofilament, SNI mice exhibit prominent hypersensitivity beginning POD 1 and continuing through at least POD 49 compared to sham or naïve animals (***p<0.005). Treatments were compared across time using a 2 way-ANOVA with Bonferroni post hoc tests. Error bars for all three graphs indicate S.E.M.