Spatial distribution of pain-potentiated synapses in hippocampal slices from naive, 2%DMSO + BV and RAPA + BV groups. (A) Averaged number of fEPSPs reliably evoked at >20% baseline ("large" fEPSPs) across the 8 × 8 array with increasing stimulus intensity. Compared to the 2%DMSO + BV group, the number of large fEPSPs in the RAPA + BV treated group was significant lower at each suprathreshold stimulus applied (30 - 199 μA). (B) Stimulus intensity-network response function curves in slices from 2%DMSO + BV and RAPA + BV groups. Individual fEPSP in DG (upper) and CA1 (lower) area increased in amplitude (a, b) or slope (c, d) at greater stimulus intensity. The input-output (I-O) function curves of the hippocampal network response were right-shifted in the RAPA + BV treatment group compared to the 2%DMSO + BV treatment group. The data were showed as means ± SD (n = 8). *P < 0.05, **P < 0.01 RAPA + BV group vs. 2%DMSO + BV group.