The total distance and region-specific distances traveled in the OF by naive, 2%DMSO + BV, and RAPA + BV groups. (A) The total distance travelled in the OF after 10, 20, 30, 40, 50 and 60 min. (B) The total distance over an entire hour. (C) The fold of distance traveled in the center, corner, or side regions of the OF to the total distance traveled (an index of place preference) for naive, 2%DMSO + BV, and RAPA + BV groups. (D - G) Differences in (D) number of hole explorations, (E) average speed, (F) number of locomotor events, and (G) time spent in locomotion for naive, 2%DMSO + BV, and RAPA + BV groups. The data were showed as means ± SD (n = 10). *P < 0.05, **P < 0.01 vs. naïve group; #P < 0.05, ##P < 0.01 RAPA + BV group vs. 2%DMSO + BV group.