Leptin and OB-Rb were upregulated in a time dependent manner in L4-6 DRG of CCI rats. (A) The expression of leptin mRNA and OB-Rb mRNA was increased in the DRG of CCI rats. Leptin and OB-Rb mRNAs were detected by RT-PCR at days 1, 7, 14 and 21 after CCI. (B) Leptin and OB-Rb protein were increased in the DRG of CCI rats. Leptin and OB-Rb protein were detected by Western Blot analysis at days of 1,7,14 and 21 after CCI. Data are shown as mean ± S.E.M, n = 6. *P < 0.05, #P < 0.01 and @P < 0.001 vs sham group.