Skip to main content
Figure 3 | Molecular Pain

Figure 3

From: Intrathecal leptin inhibits expression of the P2X2/3 receptors and alleviates neuropathic pain induced by chronic constriction sciatic nerve injury

Figure 3

Leptin attenuates the expression of IL-6, TNFα, and the P2X 2 and P2X 3 receptors in L4-6 DRG of CCI rats. (A) Leptin decreased the expression of P2X2, P2X3 receptors, IL-6 and TNFα mRNAs in a dose dependant manner. Leptin was intrathecally delivered once daily for 6 days beginning 7 days after CCI. The rats were sacrificed 24 h after the last leptin administration. The expression of P2X2 and P2X3 receptors mRNAs, and IL-6 and TNFα mRNAs were detected using RT-PCR. (B) Leptin decreased the expression of P2X2, P2X3, IL-6 and TNFα protein in a dose dependant manner. Leptin was delivered intrathecally once daily for 6 days beginning 7 days after CCI. The rats were sacrificed 24 hour after the last leptin administration. The expression of P2X2, P2X3, IL-6 and TNFα protein was detected by Western Blot. Data are shown as mean ± S.E.M, n = 6. *P < 0.05, #P < 0.01 and @P < 0.001 vs CCI + Vehicle group.

Back to article page

Molecular Pain

ISSN: 1744-8069

Contact us