Leptin administration decreases the immunoreactivities of P2X
receptors in L4-6 DRG of CCI rats. Leptin administration decreased the immunoreactivity of P2X2(A) and P2X3(B) receptors within the DRG of CCI rats. P2X2 and P2X3 receptors immunoreactivity (brown) was detected in DRG neurons of sham, CCI + vehicle, CCI + Leptin (10 μg), CCI + Leptin (50 μg) and CCI + Leptin (200 μg) treatments. Leptin was intrathecally delivered once daily for 6 days beginning 7 days after CCI. The rats were sacrificed 24 h after the last leptin administration. Arrows indicate P2X2 and P2X3 receptor positive neurons. Scale bar is 20 μm. Data are shown as mean ± S.E.M, n = 5. *P < 0.05, and #P < 0.01 vs CCI + Vehicle group.