Figure 1
From: Activation of cyclin-dependent kinase 5 mediates orofacial mechanical hyperalgesia
Analysis of the p35 expression profile in transgenic p35 (Tgp35) mice. The p35 expression levels and Cdk5 activity in the trigeminal ganglia, brainstem, and brain of the transgenic p35 mice: (A) q-PCR analysis revealed significantly enhanced levels of p35 mRNA in the trigeminal ganglia, brainstem, and the brain of the Tgp35 mice. Each data set was normalized to the expression seen in control wild-type animals. The results obtained from four different animals are expressed as mean ± SEM and analyzed by an unpaired t-test (***p < 0.001). (B) Representative Western blots showing p35 protein levels from Tgp35 tissue lysates together with corresponding densitometric analysis. Data were normalized to the levels of p35 in wild-type controls, and are presented as mean ± SEM (unpaired t-test, ***p < 0.001). (C) Cdk5 activity in the trigeminal ganglia, brainstem, and the brain of the Tgp35 mice. The data are presented as mean ± SEM and analyzed by an unpaired t-test (***p < 0.001).