Figure 3

Licking responses of Tgp35 mice during the orofacial operant assay. During the testing, the animals accessed the reward by inserting their snout through the plates with different number of wires. This was done in order to apply different force levels to the animal vibrissal pad. The number of beam breaks during the acquisition of the reward together with the licking time was measured: (A) without any stimulation, (B) using the plate with 6 + 6 wires, (C) 9 + 9 wires, and (D) 13 + 13 wires. Data are presented as mean ± SEM from four different animals measured five times in case of the baseline and three times after the induction of the trigeminal nociception (unpaired t-test, ***p < 0.001). Two sample traces show the automatic recordings of drinking behavior in a testing period of 20 minutes in a single control (upper trace) and single Tgp35 mouse (lower trace).