Figure 6

Involvement of three amino acid residues of TRPA1 in activation by Cd. Actual traces of [Ca²⁺]_i responses to Zn (3 μM, 2 min), Cd (3 μM, 2 min) and cinnnamaldehyde (CA, 300 μM) in HEK293 cells expressing wild-type (A), C641S/C1021S (B) and H983A TRPA1 (C). Concentration-response curves for Zn (D) and Cd (E) in wild-type and the two types of mutant TRPA1-expressing cells. (wild-type; n=10-40 cells, from 2 experiments, C641S/C1021S; n=13-36, from 2–3 experiments, H983A; n=17-50, from 2–4 experiments).