Figure 7
From: Involvement of TRPA1 activation in acute pain induced by cadmium in mice
Cd activates wild-type human TRPA1 but less mutant channels. Representative traces of the whole-cell currents activated by Cd (10 μM) followed by ally isothiocyanate (AITC, 50 μM) in HEK293 cells expressing wild type (A), H983A (B) and C641S/C1021S (C) TRPA1. (D) A current–voltage (I-V) curve of the Cd-activated current showing an outward rectification. Alphabet small letters (a, b) correspond to those at the time points in A. (E) Quantification of the current densities activated by Cd (10 μM) and AITC (50 μM) in HEK293 cells expressing human TRPA1; wild, a H983A mutant and C641S/C1021S mutant. Ordinates show inward current density (pA/pF) at −60 mV. Each column represents the mean with SEM. (wild-type; n=8, H983A; n=6, C641S/C1021S; n=6) **, P<0.01 vs. wild.