Figure 3
In β-arrestin 2 knockout mice CFA increased SNC80-Ca2+channel inhibition and reversed CFA-induced allodynia. A. Although basal SNC80-VDCC inhibition tended to be higher in KO mice, CFA increased SNC80-VDCC inhibition in DRG neurons above basal levels; ANOVA: F(4,58) = 9.83), ** p < 0.01 and *** p < 0.001 vs. basal/naive inhibition, shown by the dashed line. An exemplar current shows VDCCs before (1), during (2) and after (3) SNC8 (1 μM) application, vertical scalebar = 20 ms and horizontal scalebar = 0.5 pA, n = 6–14. B. The effect of SNC80 (10 mg/kg i.p.) or saline was assessed in mice which had been treated with CFA 2, 3, 7 and 14 days prior to the test. Baseline mechanical responses are represented by the dashed line. *** p < 0.001 as determined by 2-way ANOVA with a Holm-Sidak post-hoc analysis (n = 5–9 mice/group).