| Â | Naive | CFA |
|---|
Activation
| Â | Â |
Gmax | 308 ± 51 | 288 ± 41 |
Slope | 6.1 ± 0.8 | 6.5 ± 0.9 |
V1/2 | −25.2 ± 1.0 | −25.6 ± 1.0 |
Inactivation
| Â | Â |
Slope | 16.5 ± 2.5 | 9.9 ± 0.9 |
V1/2 | −13.2 ± 4.8 | −14.0 ± 1.3 |
Constitutive Inhibition
| Â | Â |
|  | 1.00 ± 0.02 | 1.01 ± 0.02 |
GABA
B
inhibition (%)
| Â | Â |
|  | 42.4 ± 3.2 | 45.5 ± 4.3 |
- Patch-clamp recordings under voltage-clamp conditions were used to examine Ca2+ channel function in medium to large-sized L4-L6 DRG neurons from naive (61± 5 pF) and CFA (64 ± 6 pF) treated mice. CFA did not alter the maximal current amplitude (Gmax; pA/pF; F(13, 234) = 0.21), the kinetics of channel activation (p > 0.05, F(13,126) = 0.04), steady-state inactivation ( p > 0.05, F(12,2040) = 1.3), constitutive current inhibition (p > 0.05, t14 = 0.89) or GABAB inhibition of the Ca2+ channels (p > 0.05, t12 = 0.59). V1/2 = half-maximal potential of the conductance-voltage relationship.
