Skip to main content
Figure 3 | Molecular Pain

Figure 3

From: Attenuation of inflammatory and neuropathic pain behaviors in mice through activation of free fatty acid receptor GPR40

Figure 3

Carrageenan-, CFA- or SNL-treatment upregulates expression level of GPR40. Representative western blots showing that the levels of GPR40 protein expression in the spinal cord (A, C, E) and dorsal root ganglia (DRGs; B, D, F) were upregulated by carrageenan (A, B), CFA (C, D) or SNL (E, F) treatment. L3-5 spinal segments and DRGs ipsilateral to carrageenan (Car) or CFA, or both ipsi- and contralateral to SNL treatment were dissected 6 hours after Car, 3 days after CFA and 2–3 weeks after SNL treatment, respectively. As a control, saline (Sal) and incomplete Freund’s adjuvant (IFA) were injected instead of Car and CFA, respectively, and for SNL, samples after sham operation (Sham) were employed. The GPR40/β-actin ratio of each control was set at 1 for quantification. Fold change of GPR40, expressed as mean ± SEM, was shown in the graph to the right or beneath each gel image. * P < 0.05 and ** P < 0.01 (Student’s t-test).

Back to article page