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Figure 4 | Molecular Pain

Figure 4

From: Attenuation of inflammatory and neuropathic pain behaviors in mice through activation of free fatty acid receptor GPR40

Figure 4

Effects of peripheral inflammation or spinal nerve injury on the GPR40 expression in the superficial spinal dorsal horn. Double-staining for GPR40 (red) and NeuN, GFAP or Iba 1 (green) with DAPI (blue) indicated that GPR40 immunoreactivity still mainly colocalized with a neuronal marker, NeuN (A, B, G, H, M, N), but rarely with an astrocytic marker, GFAP (C, D, I, J, O, P) or a microglial marker, Iba 1 (E, F, K, L, Q, R) in the spinal dorsal horn even after the carrageenan (Car) (A-F), complete Freund’s adjuvant (CFA) (G-L) or spinal nerve ligation (SNL) (M-R) treatment. Lumbar spinal cords (L4-5) were dissected 6 hours after carrageenan, 3 days after CFA, and 2–3 weeks after SNL treatment. As each control, saline, incomplete Freund’s adjuvant (IFA) and sham-treatment were employed, respectively. C-17 anti-GPR40 antibody was used. Scale bar = 25 μm.

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