Figure 6

Effects of MEDICA16 on inflammatory and neuropathic pain-like behaviors. (A) Effect of intrathecal (i.t.) injection of MEDICA16 on carrageenan (Car)-induced thermal hyperalgesia. The GPR40 agonist, MEDICA 16, was injected 6 hours after carrageenan injection. (B, C) Effects of intrathecal injection of MEDICA16 on CFA (B)- and SNL (C)-induced mechanical allodynia. Paw withdrawal latency to thermal stimuli (A) or paw withdrawal threshold to mechanical stimulation (B, C) are plotted against the time after carrageenan injection into a hindpaw (A) or after intrathecal injection of MEDICA16 with or without GW1100. Data are mean ± SEM. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, compared with pre-drug (at 6 hours in A and at 0 hour in B and C) data (one-way ANOVA followed by Dunnett’s post hoc test). ^# P < 0.05, ^## P < 0.01, ^### P < 0.001, compared with the 30 pmol group in (A) and the 10 pmol group in (B) and (C) (Student's t-test).