Figure 5

Continuous artemin injection into the plantar surface caused mechanical and heat hyperalgesia. A-B: Artemin (100 μg/ml, 100 μl/injection, PBS control n = 8) was injected into the planter surface only once and mechanical (A) and thermal (B) thresholds were tested after 5 days. A single dose of artemin, however, did not induce either mechanical or thermal hyperalgesia. C-D: Artemin (20 μg/ml, 100 μl/injection, PBS control, n = 8 each groups) was injected once a day for 5 consecutive days, resulting in significant changes in mechanical (C) and heat (D) thresholds, *p < 0.05; **p < 0.01; ***p < 0.001versus PBS group (two-way repeated ANOVA followed by Bonferroni post-test).