Figure 6

Dysfunction of glial glutamate transporters contributes to the development of paclitaxel-induced acute allodynia. (A): Bar graphs show percentage changes of glutamate uptake in the spinal dorsal horn after paclitaxel (2 ng/ml) was applied onto the intact spinal cord for 30 min. (B): Paclitaxel reduces GLT-1 and GLAST protein expressions in the cell surface. Samples of GLT-1 and GLAST protein expressions in the cell surface (membrane) and cytosol in spinal dorsal horn slices treated with paclitaxel and vehicle for 15 minutes are shown. Bar graphs show the mean (+S.E.) relative density of GLT-1 and GLAST in the plasma membrane to EGFR, and the mean (+S.E.) relative density of GLT-1 and GLAST in the cytosol to α-tubulin in each group. Number of animals included in each group for the analysis is shown in each bar. ***P < 0.001.