Effect of TCC inhibitor on neuronal activity of ACC pyramidal cells in CCI rats. A, Voltage traces showing the firing of ACC pyramidal cells in Saline (black) group and NNC 55–0396 (red, 0.1 μg/kg) group before and 30 min after drug application by in vivo patch-clamp recording. Gray dash lines indicating the potential level of −60 mV. Scale bars: 10 mV and 1 sec. B, Effect of NNC 55–0396 focal application on frequency of action potentials in ACC pyramidal cells. The frequency of action potential was sorted out from every time frame of 10 min. The drug application (▲) was performed after 10 min base recording. Data are presented over time as normalized values. *P < 0.05, **P < 0.01 compared to the Saline group. C, Effect of NNC 55–0396 focal application on frequency of burst events in ACC pyramidal cells. *P < 0.05, compared to the Saline group.