Effect of ACC microinjection with TCC inhibitor on mechanical and thermal allodynia. A, An example of microinjection site in an ACC slice with hematoxylin and eosin staining. The area in red dashed line indicating ACC, and the black arrow indicating lateral ventricle (LV). B
, Experimental time schedule. D0 refer to the day of CCI surgery. C-D, Assessing the effect of CCI surgery. The stuffed bars representing the values before CCI surgery, whereas the hollow bars representing the values on D7. ***P < 0.001 compared to the values before CCI surgery. E-F, Comparison of the effect of Saline and NNC 55–0396 (0.1 μg/kg) microinjection on mechanical and thermal allodynia. Black symbol representing the Saline group, and red symbol representing NNC 55–0396 group. The drug effect was investigated 30 min after ACC microinjection on D10. **P < 0.01 compared to the values before ACC microinjection. NS, not significant. G-H, Effective drug duration of NNC 55–0396 single treatment on mechanical and thermal allodynia. Horizontal ordinate representing the relative time of ACC microinjection. ▲: Drug application. *P < 0.05 compared to the values before microinjection.