Figure 2From: The absence of the leukotriene B4 receptor BLT1 attenuates peripheral inflammation and spinal nociceptive processing following intraplantar formalin injury TRPV1 and CGRP expression profiles of nociceptive neurons are unaffected by BLT1 receptor knockout (BLT1KO). (A) Transient receptor potential vanilloid 1(TRPV1)- and (B) calcitonin-gene related peptide (CGRP)-positive neurons in the L4 dorsal root ganglion (DRG). (C) Representative immunofluorescence images show the localization of TRPV1 (green) and CGRP (red) in L4 DRG neurons (magnification, 200×). Scale bar represents 50 μm. (D, E) Representative immunofluorescence images showing the localization of TRPV1 (green), CGRP (green) and NeuN (red) in the lumbar spinal cord (magnification, 100×). Scale bars represent 100 μm. (F) Quantitative analysis of TRPV1-immunopositive density. β-Actin and TRPA1 mRNA levels in the DRG (G) and spinal cord (H) analyzed by quantitative RT-PCR. (n = 5–7, a Kruskal-Wallis with Dunn’s multiple comparison test ). Data was expressed as Ct value.Back to article page