TRPV1 and CGRP expression profiles of nociceptive neurons are unaffected by BLT1 receptor knockout (BLT1KO). (A) Transient receptor potential vanilloid 1(TRPV1)- and (B) calcitonin-gene related peptide (CGRP)-positive neurons in the L4 dorsal root ganglion (DRG). (C) Representative immunofluorescence images show the localization of TRPV1 (green) and CGRP (red) in L4 DRG neurons (magnification, 200×). Scale bar represents 50 μm. (D, E) Representative immunofluorescence images showing the localization of TRPV1 (green), CGRP (green) and NeuN (red) in the lumbar spinal cord (magnification, 100×). Scale bars represent 100 μm. (F) Quantitative analysis of TRPV1-immunopositive density. β-Actin and TRPA1 mRNA levels in the DRG (G) and spinal cord (H) analyzed by quantitative RT-PCR. (n = 5–7, a Kruskal-Wallis with Dunn’s multiple comparison test ). Data was expressed as Ct value.