Effects of ONO-4057 on pain behavior in the formalin test. Time course of pain behavior in mice pretreated with intraperitoneal (i.p.) (A), intraplantar (ipl.) (C) or intrathecal (i.t.) (E) injections of ONO-4057 (* p < 0.05, *** p < 0.001 vs. vehicle). Total duration of pain behaviors in mice pretreated with i.p. (B), ipl. (D) and i.t. (F) injections of ONO-4057. (* p < 0.05, *** p < 0.001 vs. vehicle; n = 5–7; two-way ANOVA with Bonferroni post hoc tests).