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Figure 3 | Molecular Pain

Figure 3

From: Sensitization of P2X3 receptors by cystathionine β-synthetase mediates persistent pain hypersensitivity in a rat model of lumbar disc herniation

Figure 3

Potentiation of ATP-induced intracellular calcium mobilization in LDH rats. (A) Bright-field (upper) and DiI-fluorescence (lower) images of DRG neurons. A hindpaw innervating DRG neuron is shown in red (arrow). Bar = 50 μm. (B) An example trace of ATP (20 μM) -induced calcium signals in a DiI-labeled DRG neuron from a sham (upper) and LDH (lower) rat. (C) Bar graph showing a potentiation of ATP-induced Ca2+ transients in hindpaw innervating DRG neurons. NP application significantly enhanced ATP-induced Ca2+ transients of L5-6 DRG neurons when compared to the sham group (sham, n = 46 cells, LDH, n = 46 cells, *P < 0.05, two sample t-test). (D) The percentage of neurons responding to ATP application was not significantly altered in LDH rats when compared to sham rats (n = 80 cells for the sham group and n = 90 cells for the LDH group, P > 0.05, χ 2 test).

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Molecular Pain

ISSN: 1744-8069

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