Figure 6

Antagonism of intrathecal injection of CBS inhibitor on LDH-induced pain hypersensitivity. (A) A single intrathecal injection of the CBS inhibitor AOAA attenuated mechanical allodynia induced by LDH. The maximal effect occurred at a dose of 10 μg/kg body weight in the present study. The AOAA-induced antinociceptive effect dissipated 1 hour after injection. n = 8 for each group, *P < 0.05 compared to Pre injection values. (B) A single intrathecal injection of the CBS inhibitor AOAA reduced the body weight bearing difference induced by LDH. The maximal effect occurred at a dose of 10 μg/kg body weight. The AOAA-induced antinociceptive effect dissipated 1 hour after injection. n = 6 for each group, *P < 0.05 compared to NS. (C) Chronic effects of AOAA injection (10 μg/kg, once every day for 7 consecutive days) on mechanical withdrawal threshold. Multiple injections of AOAA attenuated PWT, which lasted for 24 hours after injection. *P < 0.05 versus NS, n = 8 for each group. (D) Multiple injections of AOAA reduced the body weight bearing difference, which lasted for 24 hours after injection. *P < 0.05 versus NS, n = 7 for each group. (E) A single intrathecal injection of AOAA did not produce any effect on the time that rats remained on the bar during the Rota rod test. (F) Multiple intrathecal injections of AOAA did not produce any effect on the time that rats remained on the bar during the Rota rod test.