MeCP2 weakens pain sensitivity through central mechanism. A, The location of MeCP2 expression in DRG and spinal cord dorsal horn (DH). In DRG, all IB4 (red) positive neurons and NF200 (blue) positive neurons have MeCP2 (green) expression (upper panel). All TRPV1 (blue) positive neurons have MeCP2 (green) expression (mid panel). In DH, MeCP2 expressed more intense than other area of spinal cord (lower panel). Representative pictures show single focal plane. Scale bars, 50 μm. B, In DRG, MeCP2 protein level was downregulated after SNI. C, Relative protein levels of MeCP2 expression in DRG. D, In spinal cord, MeCP2 protein level was elevated by 3d to 7d after SNI, and then downregulated. E, Relative protein level of MeCP2 expression in spinal cord. Bars are relative protein level to control without SNI. Error bars are SEM. *p < 0.05 (t test).