Fig. 1

Effect of OSCC-released lipids on nocifensive behavior of rats. Lipid extracts using the Folch’s extraction method were made from conditioned media of (a) immortalized normal oral keratinocyte (iNOK) cells and (b). HSC2, HSC3 and HSC4 cells. 50ul of re-suspended extracts were injected into the plantar surface of rat hindpaw and nocifensive behavior was measured upto 10 min post injection. N = 6/group. Vehicle group was injected with 50ul of PBS. Media group was injected with lipids extracted from media without exposure to cells. Data are represented as mean for each group and error bars indicate SEM. Data were analyzed using two-way ANOVA with Sidak’s post-hoc test with p <0.05. Average cell numbers per well at the time of harvest of conditioned media are indicated. c AMG517 (3 mg/kg) or HC030031 (30 mg/kg) was injected subcutaneously underneath the neck and 1 hour later, lipid extract from HSC2 cells was injected in the rat hindpaw and nocifensive behavior measured for 10 min. N =6/group. Data are represented as mean for each group and error bars indicate SEM. Data were analyzed using two-way ANOVA with Sidak’s post-hoc test with p <0.05. Asterisks indicate *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Average cell numbers per well at the time of harvest of conditioned media are indicated