Fig. 2

Effect of OSCC-released lipids on thermal thresholds of rats. Lipid extracts using the Folch’s extraction method were made from conditioned media of (a). iNOK, (b). HSC2, (c). HSC3 and (d). HSC4 cells. 50ul of re-suspended extracts were injected into the plantar surface of rat hindpaw and thermal thresholds using the radiant heat test were measured up to 150 min post injection. N = 6/group. Vehicle group was injected with 50ul of PBS. Data are represented as mean for each group and error bars indicate SEM. Data were analyzed using two-way ANOVA with Sidak’s post-hoc test with p <0.05. Asterisks indicate *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Average cell numbers per well at the time of harvest of conditioned media are indicated