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Fig. 4 | Molecular Pain

Fig. 4

From: Released lipids regulate Transient Receptor Potential Channel (TRP)-dependent oral cancer pain

Fig. 4

Effect of TRP antagonists on Lipid-induced Thermal Allodynia. TRPV1 antagonist, AMG517 (100 ug) or TRPA1 antagonist HC030031 (100 ug) was injected in the ipsilateral rat hindpaw ipl 45 min prior to lipid extract injection. Thermal threshold were measured 10 min post extract injection of the ipsilateral and contralateral paws. a 50ul of re-suspended lipid extract made from HSC2 cells was injected. b 50 ul of re-suspended lipid extract made from HSC4 cells was injected. c AMG517(100ug) or HC030031(100ug) was injected in the contralateral rat hindpaw ipl and 45 min later, HSC2 lipid extract was injected in the ipsilateral paw. Thermal thresholds were measured 10 min post extract injection of ipsilateral and contralateral paws. N = 6–8 /group. Vehicle group was injected with 50ul of 10%DMSO/90 % Mineral Oil. BL = Baseline thresholds. Data are represented as mean for each group and error bars indicate SEM. Data were analyzed using two-way ANOVA with Sidak’s post-hoc test with p <0.05. Asterisks indicate *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Average cell numbers per well at the time of harvest of conditioned media are indicated. Arrows indicate the side where vehicle or drug was injected

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