Figure 4

Characterization of IPSCs induced by AITC. (A) Action of AITC (300 μM) on IPSCs in the presence of both bicuculine (20 μM) and strychinine (4 μM). Both bicuculine and strychinine completely blocked EPSCs not only in the absence of AITC but also under its action. Traces of IPSCs are shown in an expanded scale in time, control (bottom left) and under the action of both bicuculine and strychinine (bottom right). (B) A continuous chart recording of IPSCs before and during the action of AITC (300 μM) in the presence of TTX (0.5 μM). TTX abolished the AITC-induced increases in IPSC frequency and amplitude. (C) A continuous chart recording of IPSCs before and during the action of AITC in a mixture of CNQX (20 μM) and AP5 (50 μM). The AITC-induced increase in IPSC frequency and amplitude was attenuated in the presence of the drugs. (D) Summary of EPSC frequency (left) and amplitude (right) under the action of AITC in the presence of TTX (n = 5) and AITC in the presence of CNQX + AP5 (n = 5), relative to those in the control. Vertical lines accompanied by bars show SEM. Statistical significance between data shown by bars is indicated by an asterisk; *p = 0.05. n.s., Not significant.