Analysis of the response to noxious and innocuous mechanical stimuli in the presence of AITC in the normal rat. (A) Schematic diagram showing the area analyzed. Analysis of the area subtended by the baseline and border of EPSCs was done using software (Clampfit 10, Molecular Devices). (B) EPSCs elicited by pinching in voltage-clamp mode (V
H = −70 mV) in control (left). AITC (300 μM) suppressed repeated EPSCs during pinch (middle), and the barrage of EPSCs induced by pinch appeared again after washout of AITC (right), which showed no desensitization. (C) EPSCs elicited by air puff in voltage-clamp mode (V
H = −70 mV) in control (left). AITC (300 μM) suppressed repeated EPSCs during air puff (middle) and the barrage of EPSCs induced by air puff appeared again after washout of AITC (right) (D) Analysis of evoked EPSCs with noxious and innocuous stimuli to the ipsilateral hindlimb. The area significantly decreased during AITC perfusion compared with control in the absence of AITC. Similar results were obtained with pinch (n = 11) and air puff (n = 9) to the skin. *P < 0.05.