Figure 1
From: Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons
Polysulfide stimulates a subset of mouse sensory neurons. (A) The structural formula of Na2S3. (B) An image under transmitted light, and pseudocolor images; before (Pre), after the application of Na2S3 (+Na2S3, 10 μM) and KCl (+K, 80 mM). (C) A merged image of immunostaining with antibody against PGP9.5, a neural marker and of nuclear staining with Hoechst 33752. After [Ca2+]i responses were measured, cells were subjected to immunostaining.. In (B), cells with arrows (1–3) correspond to cells in the actual recordings and immunocytochemical image. Note that only K-responding cells show positive immunoreactivity to PGP9.5 (D) Circles and columns show the concentration-response curve for polysulfide-induced [Ca2+]i increases and the percentage of polysulfide-responding neurons among all neurons, respectively (a:Na2S3, b:Na2S4). The percentages of polysulfide-responding cells were calculated from the percentage obtained with each coverslip. Symbols with vertical lines show mean ± SEM (Na2S3; n = 42–74, Na2S4; n = 33–44, from 3 mice).