Figure 3
From: Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons
Inhibition of polysulfide-induced [Ca2+]i increases by TRPA1 blockers. (A) Actual recording of [Ca2+]i responses to Na2S3 (10 μM, 8 min) and KCl (K, 80 mM) in mouse DRG neurons. (B-D) The effects of ruthenium red (1 μM), HC-030031 (10 μM) and BCTC (10 μM) on the Na2S3-induced [Ca2+]i increases. Each blocker was applied 2 min before and for 4 min during application of Na2S3. (E) Summarized effects of these blocking agents. Open and filled columns show the increases of [Ca2+]i responses to Na2S3 in the absence (Control) and presence of these blocking agents, respectively. Columns with vertical lines show mean ± SEM (control; n = 201, ruthenium red; n = 32, HC-030031; n = 24, A967079 (1 μM); n = 43, BCTC; n = 43, mibefradil (10 μM); n = 44, from 3–6 mice). **P, < 0.01 vs. Control.