Figure 6
From: Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons
[Ca2+]i and current responses to polysulfide in HEK 293 cells expressing mouse TRPA1. (A) Left shows actual traces of [Ca2+]i responses to Na2S3 (10 μM) and allylisothiocyanate (AITC, 0.3 mM) in HEK 293 cells expressing mouse TRPA1 (mTRPA1-HEK) and those to Na2S3 and capsaicin (Cap, 1 μM) in HEK 293 cells expressing mouse TRPV1 (mTRPV1-HEK). Right graph shows that the concentration-response relationships for Na2S3 in mTRPA1-HEK (closed circles) and mTRPV1-HEK (open circles). Symbols with vertical lines show mean ± SEM (mTRPA1-HEK; n = 28-65 cells, mTRPV1-HEK; n = 52-53 cells, from three different transfections). (B) Representative traces of whole-cell currents activated by Na2S3 (10 μM) followed by AITC (0.3 mM) in HEK293 cells expressing mouse TRPA1. The current–voltage (I-V) curves for Na2S3 (1) and AITC (2) exhibit outward rectification. (C,a) An actual trace of [Ca2+]i response to Na2S3 (10 μM) and AITC (0.3 mM) in RIN-14B cells (upper panel). The Na2S3-induced [Ca2+]i increase is suppressed by HC030031 (10 μM, lower panel). (C,b) The concentration-response relationship for Na2S3 in RIN-14B cells (n = 95-150, from three experiments). Vertical lines for SEM are embedded in each symbol.