Multi-channel recordings of post-LTP in the adult mouse ACC. A: schematic diagram showing the location of the MED-64 probe on an ACC slice. B: light microscopy photograph showing the relative location of ACC slice and probe. The red circle indicates the stimulation site. C and D: an overview of multi-site synaptic responses recorded at baseline (C) and 2 h after TBS (D) in one slice. After stabilizing the baseline responses for 1 h, a TBS protocol was delivered to the deep layer and LTP was then monitored for 2 h. The red filled circle denotes the stimulated channel (Ch. 36). Vertical lines indicate the layers of the ACC slice. E: results of one superficial channel (Ch. 38) showing the induction of post-LTP that lasts for 2 h in one slice. F: summary of averaged data from 6 channels in the superficial layer of the same slice. G: pooled data of the superficial layer from 5 slices of 5 mice. H: pooled data of the deep layer from 5 slices of 5 mice. Insets in E and H are sample traces at the time points indicated by numbers in the graph. Calibration: 100 μV, 10 ms. Arrows in E-H indicate starting point of TBS application. Error bars in G and H represent SEM.