Minocycline does not affect pre-LTP induction in the ACC. A: results of one channel (Ch. 38) from the superficial layer of one ACC slice. After the baseline response was stabilized for 20 min, an identical LFS + ATPA protocol was applied as that in Fig. 4 but now in the presence of minocycline (Mino, 100 μM). However, minocycline could not block the pre-LTP induction. B: summary of averaged data from 8 superficial channels of the same slice. C: pooled data for the superficial layer from 5 slices of 5 mice. D: summarized data for the deep layer from 5 slices of 5 mice. Insets in A and D show representative fEPSP traces at time points indicated by the numbers in the graph. Calibration: 100 μV, 10 ms. Horizontal bars in A-D denote the period of AP5 or ATPA or minocycline application as indicated. Arrows in A-D indicate the LFS application. Error bars in C and D represent SEM.