Fig. 2
From: The opioid peptide dynorphin A induces leukocyte responses via integrin Mac-1 (αMβ2, CD11b/CD18)
Binding of the active and nonactive αMI-domain conformers to dynorphin. a, the 3D structure of active (PDB ID 1ido) and nonactive (PDB ID 1jlm) αMI-domains are shown as ribbon diagrams. The wells of microtiter plates were coated with different concentrations of dynorphin A (b) and the D100 fragment of fibrinogen (c). The active (●) and nonactive (○) recombinant αMI-domains containing GST fusion parts were added to the wells and incubated for 1.5 h at 22 °C. Bound αMI-domains were detected with an anti-GST mAb followed by an AP-conjugated secondary antibody. Data are the means of triplicate determinations, and error bars represent S.E. Representative experiments in b (n = 4) and c (n = 4) are shown. For multiple comparisons, individual concentrations were tested using t-test with p-value of 0.05 corrected by Bonferroni method and accepting values of 0.007 as significant (**). No significant difference in binding of active and nonactive αMI-domains to Dyn A was found