Fig. 5
From: The opioid peptide dynorphin A induces leukocyte responses via integrin Mac-1 (αMβ2, CD11b/CD18)
Translocation of Dyn A into cells. Mac-1-expressing (a) and wild-type HEK293 (b) cells were incubated with 20 μM and 100 μM of Dyn A for 40 min at 37 °C, fixed, permeabilized and incubated with anti-dynorphin antibodies followed by Alexa Fluor 488-conjugated secondary goat anti-rabbit antibodies. No labeling was detected in the absence of primary antibody. c, Enlarged images of Dyn A-accumulated Mac-1- and wild-type HEK cells shown in a and b. d, Quantification of fluorescent cells shown in a and b