Fig. 6
From: The opioid peptide dynorphin A induces leukocyte responses via integrin Mac-1 (αMβ2, CD11b/CD18)
Migration of Mac-1-expressing cells to Dyn A in a Transwell system. a, Mac-1-expressing HEK 293 cells (100 μl at 3 × 106 cell/ml) were added to the upper wells of the transwell chamber and their ability to migrate to different concentrations of Dyn A placed in the lower chamber was analyzed. After 16 hours at 37 °C, migrated cells were fixed, stained, and counted. Data are presented as numbers of migrated cells per field ± S.E. for five random fields per well. The figure is representative of 5 experiments. b, Mac-1-expressing or wild-type HEK 293 cells were added to the upper wells of the transwell chamber and their ability to migrate to 10 μM Dyn A placed in the lower chamber was analyzed as in A. Some Mac-1-HEK293 cells were pretreated with 20 μg/ml of mAbs 44a (anti-αM), IB4 (anti-β2) or OKM1 (non function-blocking anti-αM) or medium alone before addition to the upper wells. ***, medium alone. Data are presented as the number of migrated cells per field ± S.E. for five random fields/well from 3 separate experiments. **, p < 0.01. c, migration of TG-elicited macrophages isolated from the peritoneum of wild-type and Mac-1-deficient mice. Macrophages (3x105) were placed in the upper chamber and allowed to migrate to Dyn A (10 μM) in the lower chamber for 90 min. The number of migrated cells was determined as in A. Data are presented as number of migrated cells per field ± S.E. for five random fields per well. The figure is representative of 3 experiments