Fig. 5

Treatment with SP600125 delays tolerance to the anti-allodynic effect of morphine on responsiveness to mechanical stimulation. Cisplatin produces time-dependent sensitization to mechanical stimulation. The time course for the development of mechanical allodynia (a) in cisplatin-treated relative to saline treated-mice. Baseline paw withdrawal thresholds (b) prior to treatment with vehicle, SP600125 (3 mg/kg i.p.), morphine (6 mg/kg i.p.) or their combination. Paw withdrawal thresholds 30 min after treatment (c) with these drug combinations. Data are expressed as mean ± S.E.M. (n = 5–6 per group). *p < 0.013 vs. vehicle or saline group (ANOVA, Bonferroni post hoc); + p < 0.006 vs. morphine or morphine with SP600125 groups (ANOVA, Bonferroni post hoc); #p < 0.01 vs. SP600125 given alone (ANOVA, Bonferroni post hoc); x p < 0.001 vs. morphine with SP600125 (ANOVA, Bonferroni post hoc)