Fig. 6

Treatment with SP600125 delays tolerance to the anti-allodynic effect of morphine on responsiveness to cold stimulation. Cisplatin produces time-dependent sensitization to cold stimulation. The time course for the development of cold allodynia (a) in cisplatin-treated relative to saline treated-mice. Baseline paw latencies in response to cold stimulation (b) prior to treatment with vehicle, SP600125 (3 mg/kg i.p.), morphine (6 mg/kg i.p.) or their combination. Paw latencies in response to cold stimulation 30 min after treatment (c) with these drug combinations. Data are expressed as mean ± S.E.M. (n = 5–6 per group). *p < 0.001 vs. vehicle or saline group group (ANOVA, Bonferroni post hoc); + p < 0.004 vs. morphine or morphine with SP600125 groups (ANOVA, Bonferroni post hoc); #p < 0.0001 vs. SP600125 given alone (ANOVA, Bonferroni post hoc); x p < 0.0001 vs. morphine with SP600125 (ANOVA, Bonferroni post hoc); ^p < 0.0001 vs. SP600125 alone or morphine with SP600125 groups (ANOVA, Bonferroni post hoc); t p < 0.001 vs. vehicle or morphine (ANOVA, Bonferroni post hoc)