Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR (a, b) and western blot (c, d) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product (upper panels) and immunoblot (lower panels) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.