Spinal nerve injury increases anoctamin-1 expression in the spinal cord and DRG. RT-PCR (a, b) and western blot (c, d) analysis of anoctamin-1 (Ano-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Insets show a representative band of the PCR product (upper panels) and immunoblot (lower panels) obtained with the specific anoctamin-1 and β-actin primers or antibodies, respectively. *Significantly different from the S group (P < 0.05), #significantly different from the Na group (P < 0.05), as determined by one-way ANOVA, followed by the Student–Newman–Keuls test. Note that spinal nerve injury enhanced Ano-1 mRNA and protein expression in spinal cord and DRG whereas that surgery increased Ano-1 expression in sham (S) animals in spinal cord but not DRG.