CaCCs inhibition reverses spinal nerve injury-induced rise in anoctamin-1 expression. Western blot analysis of anoctamin-1 (Ano-1) at the ipsilateral dorsal portion of the spinal cord (SC, a) and DRG (b) obtained from neuropathic rats with repeated intrathecal administration of vehicle (V), CaCCinh-A01, T16Ainh-A01 or NFA. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Insets in a and b show a representative blot obtained with anoctamin-1 and β-actin primary antibodies. *Significantly different from the V group (p < 0.05), as determined by one-way ANOVA, followed by the Student–Newman–Keuls test. Note that CaCCs inhibitors reduced spinal nerve injury-induced rise in anoctamin-1 expression.